Hi- 

 

I am using lumi to process data from Illumina HT-12 v3 Expression BeadChips.  I am slightly concerned about the average number of bead replicates present on the Illumina HT-12 chip, as opposed to the Illumina WG-6 chip.  Each bead is replicated on average 15 times on the Illumina HT-12 chip, as compared to an average of 30 times on the HumanWG-6 chip.  

 

Would you still suggest using VST with the Illumina HT-12 chip data, given the number of bead replicates?

 



Thanks for your help.

 

Christina Markunas



cam27@duke.edu

Graduate student

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