Hi -

I'm trying to annotate a custom cDNA array, available from GEO
(specifically, GDS1761).  It uses a custom annotation (GPL1290) which
provides the EST's that were mapped to the different spots, i.e. the first 5
rows are:
ID      NAME    CLONE_ID        5ACC    3ACC    GB_LIST
1       SID W 60204, Homo sapiens C2H2 zinc finger protein pseudogene, mRNA
sequence [5':T39154, 3':T40438]     IMAGE:60204     T39154  T40438
T39154,T40438
2        EST Chr.X [60298, (D), 5':T39213, 3':T40480]   IMAGE:60298
T39213  T40480  T39213,T40480
3       RPL3 Ribosomal protein L3 Chr.22 [60436, (EW), 5':T39295,
3':T40510]    IMAGE:60436     T39295  T40510  T39295,T40510
4       ESTSID  60474,  [5':T39311, 3':T40516]  IMAGE:60474     T39311
T40516  T39311,T40516
5       SID  60218,  [5':T39165, 3':T40450]     IMAGE:60218     T39165
T40450  T39165,T40450
6        EST Chr.18 [60268, (IR), 5':T39192, 3':T40467] IMAGE:60268
T39192  T40467  T39192,T40467

The T##### id's are the EST accession id's, which can be queried from NCBI,
i.e. for the first row
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=Nucleotide&term=T39213will
find one result,
http://www.ncbi.nlm.nih.gov/nucest/T39213.1?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum

On the result that's returned, you can find a genBank gi # that can be used
to find the gene annotation (in this case 646973) , but I can't figure out
any way to do this for a large number of EST accessions (>9600).

Any suggestions?

Thanks!

Peter

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