Dear list,

Soon I will receive data from the qpcr Exicon platform to analyze and I have
been playing around with HTqPCR package, however from the vignete, it seems
that is only capable of deleting with data design of one factor, how do I
handle it with a factorial design, namely how do I read the data and create
the model.matrix, the examples only consider one factor. Another question is
concerning part 5 of the vignete, how are the Confidence values estimated?
is is based in the variance of the data?

In order for you to understand my doubts here is my design is a 2x3 design,
I don't have replicates, but each file has the CT values for each gene frome
pooled RNA of 10 patients.
            factor1   factor2
file1.txt  NS        C
file2.txt  NS        H1
file3.txt  NS        H2
file4.txt    S        C
file5.txt    S        H1
file6.txt    S        H2


Thanks for your help.
with kind regards,
Andreia


-- 
--------------------------------------------
Andreia J. Amaral
Unidade de Imunologia Clínica
Instituto de Medicina Molecular
Universidade de Lisboa
email: andreiaamaral@fm.ul.pt
         andreia.fonseca@gmail.com

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