Dear R-Users,

I use home-made spotted arrays to do some research contected with alergies.
The matrix consist of : 60% of the genes are up-regulated and 40% of genes
that are down-regulated and spikes. I didn't use any genes with constant
expression. How I should analyse this experiment?  According to statistics I
should focuse on external spike  controls and compare all genes with spikes.
It is two coulour experiment.  So I have to build quite complicated
statistical model.  I'm not sure if it is a right pathway. What do you
think?

Regards,

Barbara

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