Hi  all,

I am using the microRNA Agilent platform to conduct a differential
expression analysis. Does anyone know what are the processing protocol that
is recommended by the Agilent manufacturer?

I think that we should use the Total Gene Signal (TGS), but, does Agilent
recommend any sort of normalization between arrays using the TGS, or the
Agilent's recommendation is just to compare the TGS between the experimental
conditions without doing anything else?  Does anyone know if there is a sort
of Agilent document where all of this is explained?

Thanks a lot.

pedro

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