Hello group, We are working on microarray data analysis of 35 experiments with limma. We have used normexp technique as background correction, loess as within array nomalization and quantile as between array normalization. After doing quantile normalization I found large number of outliers (small circles) with box plot analysis.I would like to know whether the outliers are relevent in data analysis should they be removed or can be ignored? Also if they are to be removed can any one please let me know how to minimize these outliers? Thankfully A.Ashwin Department Of Biotechnology Manipal Life Science Center Karnataka INDIA [[alternative HTML version deleted]]