On Fri, Mar 27, 2009 at 10:38 AM, Philip Twumasi-Ankrah <
nana_kwadwo_derkyi@yahoo.com> wrote:

> I am new to microarray analysis and need help on two fronts:
>
> 1. Can any one direct me to a resources that provide directions on a how to
> systematically conduct an analysis of differential expression data.
>
> Something like what the paper by Harrel and others "Tutorial in
> biostatistics,
> multivariate prognostic models: issues in developing models, evaluating
> assumptions and adequacy, and measuring and reducing errors" provides for
> regular biostatistical analysis
>
> 2. I have this affymetrix hg_u1332a chip data in a 22000 X 62 matrix. The
> columns are different patients and I am seeking suggestions on analysis. My
> goal is to report on informative genes relative to disease under study.
>

Hi, Philip.  If you take a look at the Bioconductor website, there are a
number of resources in the form of books that are not TOO expensive.  Those
are a good place to start.  Also, you might notice that each of the
bioconductor packages has at least one "vignette", sort of like a manual,
that describes how to use the various packages; these are also fantastic
sources of information.  Finally, for differential expression, there are a
number of packages in bioconductor that can do what you want, but you might
want to start with the limma package.

And just a comment on what you have so far:  a matrix is a poor
representation of microarray data.  You might want to look at the affy and
Biobase packages for the bioconductor approach to dealing with affymetrix
data.

Sean

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