On Tue, Jan 20, 2009 at 10:18 AM, John Antonydas Gaspar <
gasparj@uni-koeln.de> wrote:

> Dear Sir / madam,
>
> I wish to have an understanding of what is One class data with cl<-
> c(1,1,1,1,1)
> in SAM and Rankproduct.
>
> I have 5 Biological replicates for a sample and I wish to apply a test to
> find
> out the significant genes. As said here no control group but only 5
> biological
> replicates.
>
> Average intensity of each gene could be calculated then what can I
> do........
>
> Kindly help me out with an answer, I am just a beginner and if there is no
> sense
> in my question kindly point out that also.
>

Unfortunately, I think your experimental design leaves a bit to be desired.
Microarrays do not measure absolute expression and are only useful for
measuring relative expression, in general.  So, you need a comparison group
in order for your data to have any real meaning.

Sean

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