Hi,

  I am using *beadarray* package to analyse the microRNA expression
profiling panels (Illumina, Human, http://www.illumina.com/pages.ilmn?ID=240)
beginning from the bead-level TIFF/txt files.

  First question is about annotation of the probes: the annotation file can
be downloaded at http://www.switchtoi.com/annotationfiles.ilmn, which is a
tab-delimited file containing annotations of miRNAs. It seems that the
column 'Array_Address_Id' is actually the Probe_ID saved in the txt/csv
files, which should be used to annotate the probes. Am I right?

  Another question is the 'Detection' method in *beadarray *package, which
tells the probability that the probe are detectable against backgrounds, is
not available when I started from the bead level. Then how could I find this
threshold out? By looking at the 'AveExprs' column with 'topTable' method I
am not sure what value I should take.

  Thank you for your help,

Yours,
David

-- 
Cheers,
David

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