I'm guessing this question will show my naivete, but I wanted to see if
anyone could point me in the right direction.

 

Let's say you have a gene like KRAS that gets a DNA point mutation
(missense) in a tumor cell, and that cell clonally expands. Then let's say
you use a microarray to try to detect mRNA expression for that gene (and
others).

 

Will the mutation affect your ability to detect mRNA expression for KRAS? Or
in other words, will the expression level be detected as lower because the
RNA sequence was different than the reference sequence on the chip?

 

Any way to get around this?

 

Know of any papers that discuss this?

 

Thanks in advance.

 

Regards,

-Steve

 

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Stephen Piccolo

 

PhD Student

Department of Biomedical Informatics, University of Utah

26 South 2000 East

Suite 5700 HSEB

Salt Lake City, UT 84112-5750

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