On Thu, Nov 20, 2008 at 5:37 AM, John Antonydas Gaspar <gasparj@uni-koeln.de
> wrote:

> Dear Sir,
>
> I am in need of your help to decide which test would be an appropraite in
> this
> case.
>
> I am involved in the data analysis of the out put from Illumina.
> I have the following experimental design
>
> Array A : TBVII Treat 01
> Array B : TBVII Treat 02
> Array c : TBVII Treat 03
> (they are biological replicates)
> Array D : SSC15 -01
> (just a sample without replicate)
> Array E : TBVII control 01
> Array F:  TBVII control 02
> (They are technical replicate)
>
> I understand that this is a Bad experimental design but a group wants to do
> have
> some preliminary information to have from this.
>
> Kindly help me out to decide what sort of statistical test I can apply for
> the
> anlaysis. There is sample without any replicate. It would be of great help
> since I am only a beginner for this type of analysis.
>

I would simply make a heatmap of the top 200 or so varied genes or those
with the largest fold changes.  There are not meaningful statistics that can
be done with an n of 1.

Sean

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