Dear all,

Im sorry if this is a question not directly related to using  
bioconductor packages, but at least I used them for the analysis that  
I need a reference to ;o)

I have a biopsy related project with 63 unpaired biopsies in total  
and two groups where I cannot find any differences using limma,  
probably due to tissue heterogeneity. I wonder if there is anyone who  
has published a paper perhaps with different mixes of cell lines and  
compared that to clean comparisons between the cell lines, in order  
to draw conclusions about what level of heterogeneity that is  
possible to detect using e.g. Affymetrix/Agilent or some other  
platform? Im going to try to publish a negative result and it would  
be great if anyone has tried to do some quantification around this  
problem.

I have looked around and I found the paper by Holloway et al,  
"Statistical analysis of an RNA titration series evaluates microarray  
precision and sensitivity on a whole-array basis", which is a really  
good paper but here they only have two cell lines and it would be  
nice to have a look of what it looks like in mixtures.

best regards,

// Johan



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