HuEx 1.0 st v2  

Two replicates each at 0h 3h 6h 12h 24h 48h

 

 

I've extracted my summarized data from aroma.affymetrix as a data frame,
I now need to import it into limma and normalize to the 0h time point.
My ultimate goal is to return a gene list and heatmap that pass my
filters, say p<=.05 and fc>=1.5  

 

My main question however, is if there is a function in limma or any
other package that will perform this normalization to the 0h time point
and create ratios of the form 

(Time x)/(time 0)

 

Thank you

Ray


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