Hello,

 

I will be be obliged if someone can reply to this query:

 

I have a question related to expresso and limma package.

 

I have mas5.0 normalized data and I do not have the raw .CEL files.

I want to use limma on the mas5.0 data to find differentially expressed
genes. 

 

I read in the journal publication "affylmGUI: a graphical user interface for
linear modeling of single channel microarray data", 

that in the non-standard way, one can use the normalize raw .CEL files by
mas5.0 methiod using expresso() function with parameters

and then storing the result of this procedure in an object called
NormalizedAffyData will ensure that it is recognized by affylmGUI for the
purposes of creating plots and

fitting linear models.

 

I created an eSet using my mas5.0 data but it wasnot recognized by
expresso(), since it is not a affybatchObject.

 

Can anyone suggest me how I can import mas5.0 normalized data into expresso,
so that after I process the data there, I can import it into limma?

 

 

Thanks

manisha

 

 

 


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