Hi,

 

I'm sorry I have to send another query. I am still trying to load my agilent
FExtraction data using:

> RG<-read.maimages("targets.txt", source="agilent",
columns=list(gBGMeanSignal="F635", gBGMeanSignal="F532",
bBGMedianSignal="B635 Median", bBGMedianSignal="B532 Median"),
annotation=c("Block", "Column", "Row", "Name", "ID"))

 

But I keep getting errors like:

Error in read.maimages("targets.txt", source = "agilent", columns =
list(gBGMeanSignal = "F635",  : 

columns must specify foreground G and R

 

My data is read as an agilent single colour and therefore I've only a Cy3
output. How then do I define the foreground for R? also what does F635 and
B532 means? 

 

Wendy

 


	[[alternative HTML version deleted]]

