Hi Sean,

I was trying to read a single channel Agilent data which has been extracted
using agilent feature extraction software.
I tried limma using read.maimages giving source as agilent, as the format
itself is different it failed to read. So I made log ratio of the test Vs
control in the log ratio column and the test median signal assigned to
rMedianSignal and the control assigned to gMedianSignal. So this text file
has recognized by read.maimages source as agilent.
I have performed a loess normalization using normalizeWithinArrays(without
background subtraction).
In this scenari what will be the appropriate approach?

Abhilash

On Sat, Apr 19, 2008 at 9:53 PM, Sean Davis <sdavis2@mail.nih.gov> wrote:

> On Sat, Apr 19, 2008 at 10:05 AM, Abhilash Venu <abhivenu@gmail.com>
> wrote:
> > Hi all,
> >
> >  Which package will be good for analyzing Agilent single channel data?
>
> Hi, Abhilash.  Can you be more specific about what you have tried and
> where you have looked?  Then, it will be easier to give you direction.
>
> Sean
>



-- 

Regards,
Abhilash

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