
The Reference in qPCR - Academic & Industrial Information Platform

Dear researcher,
dear Gene Quantification page reader,


The Gene Quantification page - www.Gene-Quantification.info - describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & qRT-PCR. It presents a lot of applications, chemistries, methods, algorithms, cyclers, kits, dyes, analysis methods, meetings, workshops, and services involved. Commercial and academic institutions can present their qPCR tools right here  =>  http://qpcrplatform.gene-quantification.info/

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Our newsletter informs about the latest news in quantitative real-time PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene Quantification homepage. The focus of the last newsletter issue was:

=>  Copy Number Variation
=>  new webinars online
=>  new application workshops in 2008

   http://qpcrnews.gene-quantification.info/

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Data Analysis and BioInformatics in real-time qPCR

Bioinformatics is a multidisciplinary approach to discribe, model and understand biological processes on basis of information on genes, proteins and metabolism. It uses computers, data bases and algorythms to link information and translate it back into biology, physiology or pathophysiology.
BioInformatics => Database Management Systems, Data Mining, Sample Tracking, Information Management, Data     Acquisition, Data Analysis, Statistics, Pattern Recognition & Classification, Simulation & Modeling
Bioinformatics initially centered on sequence and genome analysis but now the extensive use of microarrays, mass spectrometry, qPCR and qRT-PCR, has stimulated bioinformatic work in data acquisition, signal processing, and data mining. Also, simulation and modeling are becoming increasingly important areas of focus in bioinformatics which finally will lead to a new level of understanding the networks in the metabolism: Genomics, Transcriptomics, Splicomics, Proteomics, Metabolomics, etc.

http://bioinformatics.gene-quantification.info/

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The quantification strategy in real-time RT-PCR is the principal marker in gene quantification. Generally two strategies can be performed in real-time RT-PCR. The levels of expressed genes may be measured by absolute or relative quantitative real-time RT-PCR. Absolute quantification relates the PCR signal to input copy number using a calibration curve, while relative quantification measures the relative change in mRNA expression levels. The reliability of an absolute real-time RT-PCR assay depends on the condition of ‘identical’ amplification efficiencies for both the native target and the calibration curve in RT reaction and in following kinetic PCR. Relative quantification is easier to perform than absolute quantification because a calibration curve is not necessary. It is based on the expression levels of a target gene versus a housekeeping gene (reference or control gene) and in theory is adequate for most purposes to investigate physiological changes in gene expression levels. The units used to express relative quantities are irrelevant, and the relative quantities can be compared across multiple real-time RT-PCR experiments.

http://strategy.gene-quantification.info/

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With this page and all the presented tools we will help you with to find the right information about qPCR and related topics in Molecular Biology in the literature and in the World Wide Web:
New publications / Protocols updated ! / Alets / Feeds / Books / Forums / E-mail Services / Directory

http://infoportal.gene-quantification.info/

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TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops, a 3-day qPCR Core Module and a 2-day qPCR Biostatistics Module.  All courses are held regularly in Göteborg, Sweden, in English and in Freising-Weihenstephan, Germany, in German and English, and in Prague, Czech Republic in English and Czech.

http://tataa.gene-quantification.info/

Course Occasions 2007 and 2008:

3-day qPCR Core Module  (Mon. - Wed.)    and     2-day BioStatistics Module (Thu. - Fri.)

=> 3rd - 7th March 2008  (in Freising, Germany, English language)
=> 5 - 9th May 2008 (in Freising, Germany, Kurs wird in DEUTSCH gehalten, German language)
=> 7 - 11th July 2008  (in Freising, Germany, English language)

Please register here  =>  http://www.tataa.com/Courses/Courses.html

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Please send the mail to further scientists and friends who are interested in qPCR !


Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages
http://www.Gene-Quantification.info


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The qPCR NEWS and the Gene Quantification Pages are educational sites with the only purpose of facilitating access to qPCR related information on the internet.  The qPCR NEWS and the Gene Quantification Pages are edited by Michael W. Pfaffl and powered by BioScience Events.  Copyright © 2005 - 2007  All rights reserved.  Any unauthorized use, reproduction, or transfer of this message or its contents, in any medium, is strictly prohibited. Disclaimer & Copyrights are displayed on the homepage  www.gene-quantification.com    To subscribe or change your e-mail address in qPCR NEWS, and if you would like to receive future issues FREE of charge, please send an e-mail with the subject SUBSCRIBE to  mailto:newsletter@gene-quantification.info?subject=SUBSCRIBE    To unsubscribe from the qPCR NEWS, please send an e-mail with the subject UNSUBSCRIBE to  mailto:newsletter@gene-quantification.info?subject=UNSUBSCRIBE

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