Hello,

 

I have some double channel array experiments (see targets file below).  For
some of the arrays I do not expect equal amounts of labeled dye
(specifically the channels with the 'nothing' cDNA have low intensities.
Basically I want to normalize between arrays, but only using the channels
that had 'chlor' treatments hybridized to them.  Is there an easy way to do
this?

 

Thanks,

 

Alison

 

 

             SlideNumber         FileName      Cy3      Cy5      Date

july11_2812          281  july11_2812.gpr           starved 11-Jul-07

july11_7261          726  july11_7261.gpr    chlor    chlor 11-Jul-07

july11_7322          732  july11_7322.gpr    chlor    chlor 11-Jul-07

july11_0740          740  july11_0740.gpr    chlor    chlor 11-Jul-07

797scan2bckp         797 797scan2bckp.gpr methanol    chlor 12-Mar-06

729Sscan2            729    729Sscan2.gpr    chlor methanol 12-Mar-06

723scan1             723     723scan1.gpr    chlor methanol 12-Mar-06

716scan1             716     716scan1.gpr  nothing    chlor 12-Mar-06

847scan1             847     847scan1.gpr    chlor  nothing 27-Feb-06

841scan2             841     841scan2.gpr  nothing    chlor 27-Feb-06

830scan3             830     830scan3.gpr methanol    chlor 27-Feb-06

791scan2bckp         791 791scan2bckp.gpr    chlor methanol 27-Feb-06

784scan2             784     784scan2.gpr    chlor methanol 27-Feb-06

778scan2             778     778scan2.gpr methanol    chlor 27-Feb-06

853scan1            1853     853scan1.gpr  nothing methanol 25-Feb-06

451scan2            1451     451scan2.gpr methanol  nothing 25-Feb-06

836scan1             836     836scan1.gpr    chlor methanol 14-Feb-06

829scan1             829     829scan1.gpr methanol    chlor 14-Feb-06

846scan1             846     846scan1.gpr methanol    chlor 14-Feb-06

 

******************************************
Alison S. Waller  M.A.Sc.
Doctoral Candidate
awaller@chem-eng.utoronto.ca
416-978-4222 (lab)
Department of Chemical Engineering
Wallberg Building
200 College st.
Toronto, ON
M5S 3E5

  

 


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