I am reading my imagene intensity files in Limma.  The following example is given.



files <- targets[,c("FileNameCy3","FileNameCy5")]



My statement reads



files <- targets[,c("c:/Documents and Settings/HP_Administrator/Desktop/2004 coho t48h quantified files/2007 09 19 slide 226 2004 coho t96 Cyanine 3.txt","c:/Documents and Settings/HP_Administrator/Desktop/2004 coho t48h quantified files/2007 09 19 slide 226 2004 coho t96 Cyanine 3.txt")]



I get the following error message:



Error in `[.data.frame`(targets, , c("c:/Documents and Settings/HP_Administrator/Desktop/2004 coho t48h quantified files/2007 09 19 slide 226 2004 coho t96 Cyanine 3.txt",  : 
  undefined columns selected



What am I doing wrong and how do I fix it



Sally



PS: the following statment below worked just prior to the above statement



> Target <- readTargets("c:/Documents and Settings/HP_Administrator/Desktop/limma practise files")

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