Hello,

Sometimes GPR headers become corrupted for reasons unknown to me (I know it
happens though).  In particular, the second line of the gpr output has a
value that corresponds (I think) to the start of the data, and limma needs
this value to begin reading data.  If this value is incorrect, then the file
cannot be read in.  This happened to me, I fixed that line, and it worked
just fine thereafter.

In fact, I've had a few occasions where limma would not read in a file, and
I traced them all back to the GPR files.  That would be a good place to
start (unless someone else here has the magic answer).

Cheers,

Adrian



On 6/6/07, Ziping Zhang <zipingcn@msu.edu> wrote:
>
> Dear Sir,
>
> I am using your limmaGUI for microarray analysis. My data is collected by
> GenPix. limmaGUI was runing well last week. However, today it shows me
> these
> information when I was running a new set of data:
>
> limmaGUI was unable to read the image processing files listed in the
> Target
> files
> Error in assign("RG",RG,limmaGUIenvironment):object"RG" not found
> Error in MA.RG(RG):Object doesn't contain R and G components
> Error in assign("MAraw",MAraw, limmaGUIenvironment): object "MAraw"
> not found
> Error in assign("MA", MAraw, limmaGUIenvironment): object"MAraw" not
> found
>
> Would you please kindly help me figure out how to solve the probelem.
> Thanks
> a lot.
>
> Zhang, Ziping (Ph.D.)
> Department of Fisheries and Wildlife
> 13 Natural Resources Building
> 158 Giltner Hall
> Michigan State University
> East Lansing, MI 48824
> Tel: 517-4323314
> Fax: 517-4321699
>
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