 Thanks for your response and sorry for my oversight
This is the info session:

R version 2.4.1
i386-pc-mingw32

attached base packages:
[1] "grid" "tools" "stats" "graphics" "grDevices" "utils" "datasets"
"methods" "base"

other attached packages:
prada "1.10.1"  geneplotter "1.12.0"  annotate "1.12.1"  RColorBrewer "0.2-3"

Biobase "1.12.2"


I´m tried Proceed and it's good for clean a population using two new
variables for gating, for example trying first FL2 vs FL3 and then proceed
with SSC vs FL3. In the second plot only appear those events that are gated
by the first gate and it's easy to clean the values that are out of the
population. But when i'm trying to use the option proceed with the same
channels, i can't apply the distinct gates with applyGate( pico.sub,
f1[[1]]) for example,  to
get the events in the first gate.

Thanks for your response


2007/4/24, Nolwenn LeMeur <nlemeur@fhcrc.org>:
>
> Hi Florencio,
>
> Could you tell me the version of your prada package (sessionInfo)?
> I tried with one of my file and  I got the same error message when
> using combine
> however it seems to worked when I am using Proceed instead of Combine.
> Have you tried?
> Maybe you should directly contact Florian Hahne the author and
> maintainer of the package.
>
> Cheers,
> Nolwenn
>
>
> Florencio Gonzalez wrote:
>
> > Thank you for the information. I'll give a try the new package. Just a
> > question about drawGate.
> > How can i get two or more populations from a file with the same
> > drawgate command? I see an option when creating the gate for combine
> > the gating with another but i get an error
> >
> > > pico <- read.fcs("0103")
> > > pico.sub <- pico[, c(2,4,5)]
> > > f1 <- drawGate(pico.sub)
> > Select first variable for gating...FL2-H
> > Select second variable for gating...FL3-H
> > Type of gate? (P)olygon or (R)ectangular?p
> > Draw gate (left click: set point, right click: end drawing).
> > Determining events within gate...found 1987
> > (R)edo this gating,  (C)ombine this gating with another one ,
> > (P)roceed or (F)inish? c
> > Select first variable for gating...FL2-H
> > Select second variable for gating...FL3-H
> > Type of gate? (P)olygon or (R)ectangular?p
> > Draw gate (left click: set point, right click: end drawing).
> > Determining events within gate...Erro en validObject(.Object) :
> > invalid class "gate" object: invalid object for slot "boundaries" in
> > class "gate": got class "list", should be or extend class "matrix"
> >
> > --
> > Florencio González Blázquez
> > Instituto Español de Ocenografía
> > Centro costero de Gijón
> > Avd. Principe de Asturias 70 bis
> > Gijón. España
>
>
>


-- 
Florencio González Blázquez
Instituto Español de Ocenografía
Centro costero de Gijón
Avd. Principe de Asturias 70 bis
Gijón. España

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