Hello,

I would like to use data extracted from images scanned at 3 different 
intensities in our GenePix scanner.  There are a couple of papers 
that I could find (Lyng et al 04, Piepho et al 06) that describe 
methods to combine these data and thus help deal with problems of 
saturation and signals across the dynamic range of the scanner.

I looked for a way to do this in bioconductor, and found a post from 
Dr. Henrik Bengtsson, indicating that this was possible using the 
aroma.light package in bioconductor.  However, he indicated that this 
should be done with data from scans in which the laser intensity =was 
not changed=.

Unfortunately, my scans used two different laser intensities.

Does this invalidate using aroma.light for this purpose?  Is there 
any other Bioconductor package that could deal with my (apparently 
incorrectly obtained) data?

many thanks!
John

-- 
John Fowler				Associate Professor
Botany and Plant Pathology (BPP) Dept.  
2082 Cordley Hall                        Phone: (541) 737-5307
Oregon State University                  FAX: (541) 737-3573
Corvallis, OR  97331-2902  USA	  	 Email: fowlerj@science.oregonstate.edu
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