I have been asked to analyzed a dataset with the following design:

 

3 rat lines (P, NP, W)

2 treatments (E, H2O)

2 time points (1, 2)

2 technical replicates for each data point

 

Also, we have the ability to measure the actual blood alcohol content
(BAC) at each time point.

 

Because we will be sacrificing rats at each time point, this is not a
true repeated measures experiment as different rats will be used for
time point 1 than for time point 2.

 

The response variable, protein expression, is continuous.

 

This is a much more complicated design than I am used to working with.
Which BioC package would be the easiest to use in analyzing this data? I
am used to LIMMA, but I cannot find anything in the LIMMA vignette that
describes incorporating the continuous variable of BAC into a design.
Would MAANOVA work? Or, is there something better?

 

Thanks,

Mark

 

Mark W. Kimpel MD 

 

 

Official Business Address:

 

Department of Psychiatry

Indiana University School of Medicine

PR M116

Institute of Psychiatric Research

791 Union Drive

Indianapolis, IN 46202

 

Preferred Mailing Address:

 

15032 Hunter Court

Westfield, IN  46074

 

(317) 490-5129 Work, & Mobile

 

(317) 663-0513 Home (no voice mail please)

1-(317)-536-2730 FAX


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