What we do is to to use read.table or read.delim 
to read the data into a dataframe.

I have not found it necessary to create an 
exprSet object from the dataframe.  However, if 
you feel it is necessary, you can
create each component using the columns of the 
dataframe as needed and then set the class to exprSet.

The documentation for an exprSet is in 
Biobase.  (Note the uppercase S.)  It shows how 
the "new" command can be used to
create the exprSet once you have all the 
components.  (Components can be empty.)

--Naomi

At 10:24 PM 1/22/2007, you wrote:

>Data is in Excel from Nimblegen, single channel, not transformed but
>normalized.
>
>Thanks for your response.
>
>Sarah
>
>
>On 1/22/07 9:17 PM, "Naomi Altman" <naomi@stat.psu.edu> wrote:
>
> > What form is the data in currently?  Do you have the scanner output files?
> >
> > --Naomi
> >
> > At 05:53 PM 1/22/2007, Sarah Highlander wrote:
> >> Išm just getting started and need to create 
> an exprset. Searched the web and
> >> I canšt find an explanation. Išm working on a Mac OS 10.4
> >>
> >> Thanks,
> >>
> >> Sarah
> >> Sarah K. Highlander, Ph.D.
> >> Associate Professor
> >> Baylor College of Medicine
> >> Department of Molecular Virology and Microbiology
> >> One Baylor Plaza, MS280
> >> Houston, TX 77030-3498
> >>
> >> 713-798-6311 Voice
> >> 713-798-6802 FAX
> >> sarahh@bcm.tmc.edu
> >>
> >>
> >>
> >>         [[alternative HTML version deleted]]
> >>
> >> _______________________________________________
> >> Bioconductor mailing list
> >> Bioconductor@stat.math.ethz.ch
> >> 
> <https://stat.ethz.ch/mailman/listinfo/bioconductor>https://stat.ethz.ch/mailman/listinfo/bioconductor
> >> Search the archives:
> >> 
> <http://news.gmane.org/gmane.science.biology.informatics.conductor>http://news.gmane.org/gmane.science.biology.informatics.conductor
> >
> > Naomi S. Altman                                814-865-3791 (voice)
> > Associate Professor
> > Dept. of Statistics                              814-863-7114 (fax)
> > Penn State University                         814-865-1348 (Statistics)
> > University Park, PA 16802-2111
> >
>
>Sarah K. Highlander, Ph.D.
>Associate Professor
>Baylor College of Medicine
>One Baylor Plaza, MS280
>Houston, TX 77030-3498
>
>713-798-6311 Voice
>713-798-6802 FAX
>sarahh@bcm.tmc.edu
>

Naomi S. Altman                                814-865-3791 (voice)
Associate Professor
Dept. of Statistics                              814-863-7114 (fax)
Penn State University                         814-865-1348 (Statistics)
University Park, PA 16802-2111

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