Hi all. 

I got some facs files from a collaborator and I was glad to discover that
there was actually a package in bioconductor that could be used to analyze
facs data. But  I didn't even make it past reading the file into R. 

 

I would greatly appreciate if someone could give me a hint of what went
wrong. I tried the suggestions that were given in the help file but they
didn't seem to able to help for my specific problem.

 

> FC.FCSRobj <- read.FCS(fileName="Panel1APBMC.fcs", MY.DEBUG = TRUE)

Start/Stop:$version

[1] "FCS3.0"

 

$TextStart

[1] 318

 

$TextEnd

[1] 2972

 

$TextLength

[1] 2655

 

$DataStart

[1] 2973

 

$DataEnd

[1] 17002972

 

$DataLength

[1] 1.7e+07

 

$AnalysisStart

[1] 0

 

$AnalysisEnd

[1] 0

 

[1] "Obtaining the metadata."

Error in if (is.na(metadata.list$"$BYTEORD")) { : 

        argument is of length zero

In addition: Warning messages:

1: data length [2655] is not a sub-multiple or multiple of the number of
rows [1328] in matrix 

2: is.na() applied to non-(list or vector) in:
is.na(metadata.list$"$BYTEORD")

 

> sessionInfo()

Version 2.3.1 (2006-06-01) 

i386-pc-mingw32 

 

attached base packages:

[1] "grid"      "splines"   "methods"   "stats"     "graphics"  "grDevices"
"utils"     "datasets"  "base"     

 

other attached packages:

  rflowcyt     fields    rfcdmin     hexbin colorspace    lattice    splancs
sp       MASS KernSmooth     xtable 

   "1.0.1"     "3.04"    "1.4.0"    "1.6.0"      "0.9"  "0.13-10"  "2.01-19"
"0.8-20" "7.2-27.1"  "2.22-19"    "1.3-2" 

  survival 

    "2.28"

 

Best Regards

 

// Johan Lindberg

 

 

 

 

 

 

 


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