Hi,

Degradation plots that I have done in the past have usually
produced plots that increase towards the 3' end in a roughly
uniform manner. I have done this (breast) experiment on a new
chip which results in the folowing plot:

http://www.redbrick.dcu.ie/~osama/images/RNAdeg.jpg

This seem to be rather strange,as it peaks in intensity before
the farthest 3' end. Is there any explanation for this or is there
any documetnation of this happening elsewhere?

Thanks,

Martin

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