Dear list,

I have a very basic question regarding reading imagene files. I have a
targets.txt file as such:

SlideNumber    FileNameCy3    FileNameCy5    Cy3    Cy5
1    Cy3WT1.txt    Cy5KO1.txt    WT    Mu
2    Cy3KO2.txt    Cy5WT2.txt    Mu    WT

And when I try to read the file, everything seems to get read well except
when I try to visualize the contents, the red channel columns are named
Cy3WT1 and Cy3KO2 when they should be Cy5KO1 and Cy5WT2 (as I've pasted
below). Any help would be appreciated. Thanks!

> targets <- readTargets()
> files <- targets[,c("FileNameCy3", "FileNameCy5")]
> RG<-read.maimages(files, source="imagene")
Read header information
Read Cy3WT1.txt
Read Cy5KO1.txt
Read Cy3KO2.txt
Read Cy5WT2.txt
> show(RG)
An object of class "RGList"
$R
         Cy3WT1    Cy3KO2
[1,]   413.7954   404.293
[2,] 31634.9648 48596.723
[3,] 36108.0234 46955.476
[4,]  7986.0908  6745.707
[5,]  7147.1035 11792.948
38875 more rows ...

$G
        Cy3WT1     Cy3KO2
[1,]  1642.682   819.3275
[2,] 33933.414 46091.7421
[3,] 39531.910 43781.1132
[4,]  8942.613  7269.6206
[5,]  7607.328 12893.2929
38875 more rows ...
etc...

	[[alternative HTML version deleted]]

