Hi All,

Sorry to raise up this question again. I did search the forum but failed to
get the answer.

Can anyone has an idea what "16" means in "plier+16"?

Thanks in advance,

Frank

---------- Forwarded message ----------
From: Ariel Chernomoretz <ariel.chernomoretz@crchul.ulaval.ca>
Date: Feb 7, 2005 11:55 AM
Subject: [BioC] MAS5+32, PLIER+16 alorithms reported in affycomp
To: Bioconductor <bioconductor@stat.math.ethz.ch>

Dear list memebers,

I was looking at affycomp benchmark results and found some entries that
I do not understand.
Can anyone tell me what '+32' and '+16' mean in MAS+32 and PLIER+16?
I think they have to do with variance stabilization but I would
appreciate if anyone
could give my further details.

In addition, can anyone point me to a technical reference regarding the
Affymetrix new PLIER summarization method? Is it a whole solution, i.e.
background substraction + probe level normalization + probeset
expression estimation, or just the latter? In that case which
background and normalization steps are advice to be used with?

Thanks,


Ariel Chernomoretz, Ph.D.
Centre de recherche du CHUL
2705 Blv Laurier, bloc T-367
Sainte-Foy, Qc
G1V 4G2
(418)-525-4444 ext 46339

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