Hi, there,

 

I am using limma for some single channel microarray data. Date format
follows that suggested by user guide: probes in row and arrays in
column. Data set has no missing value and was read into R by read.table
function. Design matrix is as following:

 

            P          R

P1        1          0

P2        1          0

P3        1          0

P4        1          0

P5        1          0

P6        1          0

P7        1          0

R1        0          1

R2        0          1

R3        0          1

R4        0          1

R5        0          1

R6        0          1

 

When I ran lmFit(data, design), I got this error: 

 

> fit<-lmFit(data, design)

Error in qr(x) : NA/NaN/Inf in foreign function call (arg 1)

In addition: Warning message:

NAs introduced by coercion

 

Traceback gave this: 

> traceback()

 

3: qr(x)

2: nonEstimable(design)

1: lmFit(data, design)

 

I have converted data and design to numeric matrices but they never
worked. Could you please what's the problem? I really don't know what's
going on with it. Thanks a lot!

 

Yezhou Sun


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