Hello All,

I used vsn to normalze my one-channel cDNA microarray experiment.  I'm sorry
if this is an elementary question (I'm not a math person) but can vsn data
be interpreted in similar fashion to log2 data, e.g. 1 log vale = 2-fold
induction?  What would be the appropriate transformation to get to either
log2 or raw data from vsn data?

Briefly, what I did was to normalize using vsn, then I used SAS to run
anovas with pairwise comparisons and anova slicing.  Using the estimate
function returns estimated differences between the reported means.  I am
seeking then to bridge the gap between these estimates and actual fold
changes.  I think this can be done, but I am unsure about how to either
reverse-transform the vsn data or how to interpret it biologically (e.g. 1
log = 2x fold change)

WIth thanks

Maurice

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