Hello,
  Could anyone tell me how to go about doing an analysis for a single microarray slide using limma.
  Below is the code I used to specify the design,
  
  fit <- lmFit(MA, design=c(1))
  
  But I get the following errorr once I go to  fit <- eBayes(fit)
  
  Error in ebayes(fit = fit, proportion = proportion, stdev.coef.lim = stdev.coef.lim) : 
          No residual degrees of freedom in linear model fits
  
  I am not a statistician, so I need help to interpret the above error.
  
  Thanks and regards
  
  Ankit
  
  
  

		
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