When running 

RGb<-backgroundCorrect(RG,method="normexp")

I get the following warning at the end.

 

Corrected array 1 

Corrected array 2 

Corrected array 3 

Corrected array 4 

Warning message:

Limit of numerical accuracy reached with very low intensity or very high
background:

setting adjusted intensity to small value in: normexp.signal(mu = out$beta +
RG$Gb[, j], out$sigma, out$alpha,  

 

I have used normexp but not have seen this message.

Anyone know what might be wrong?

 

 

 

 


The background corrected values show up as:

 

$R

            098680        099711        099712        099713

[1,] 6.689116e+106 8.195925e+119 9.212567e+185 4.092258e+101

[2,] 6.689116e+106 8.195925e+119 9.212567e+185 4.092258e+101

[3,] 6.689116e+106 8.195925e+119 9.212567e+185 4.092258e+101

[4,] 6.689116e+106 8.195925e+119 9.212567e+185 4.092258e+101

[5,] 6.689116e+106 8.195925e+119 9.212567e+185 4.092258e+101

12283 more rows ...

 

$G

            098680       099711      099712 099713

[1,] 1.965623e+243 1.409119e+44 3.00506e+49  1e-06

[2,] 1.965623e+243 1.409119e+44 3.00506e+49  1e-06

[3,] 1.965623e+243 1.409119e+44 3.00506e+49  1e-06

[4,] 1.965623e+243 1.409119e+44 3.00506e+49  1e-06

[5,] 1.965623e+243 1.409119e+44 3.00506e+49  1e-06

12283 more rows ...

 

 

 

 

The original data was as follows

 

$R

     098680 099711 099712 099713

[1,]    223    238    271    247

[2,]  34997   7087  48309  63553

[3,]    320    388    930    455

[4,]    324    250    360    445

[5,]   2634   1744    870   1763

12283 more rows ...

 

$G

     098680 099711 099712 099713

[1,]   1643   2682    620    662

[2,]  62144  19362  12262  28980

[3,]   1427   1619    832    612

[4,]   2069   1343    878    663

[5,]   3531   3868   1265   1194

12283 more rows ...

 

$Rb

     098680 099711 099712 099713

[1,]    149    187    184    190

[2,]    162    203    229    197

[3,]    158    181    200    189

[4,]    158    197    180    185

[5,]    157    344    180    181

12283 more rows ...

 

$Gb

     098680 099711 099712 099713

[1,]    532    860    174    160

[2,]    551    812    198    164

[3,]    598    431    197    155

[4,]    598    448    183    151

[5,]    669    511    173    167

12283 more rows ...

 

 


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