I am interested to know how one may determine if two conditions can have
synergistic effects.  For example, lets say we expose cells to two
conditions, X and Y.  We have four microarray chips.  

 

            0          X          Y          X+Y

 

Lets suppose the following raw data

 

            8          2046     16        4096

 

Log2 of those numbers

 

            3          11        4          12

 

Log fold change vs no stimuli

 

                        8          1          9

 

Fold change

 

                        256       2          512

 

Raw change

 

                        2038     8          4088

 

 

 

If you look at the fold change one can say there is no synergistic effect
since the log fold change of 9 can be explained by the 2**8 increase and the
2**1 increase.  (or I could say the 512 comes from the 256x component and 2x
component)

 

If you look at the raw change, it appears to be synergistic since
2038+8<<<4088.

 

I am not a mathematician so I don't know which would be the proper way to
look at this.  Would an example like this be synergistic.  And what is the
best way to approach this.

 

 


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