Dear all,

 

Perhaps my question is very naive, but I need absolute expression values for use in a certain clustering program. I have cDNA arrays, which were made by loop design, meaning all the samples have been mostly related to each other, though there a few arrays with gDNA, which I'm using as a common reference. I make normalisations within array (loess method) and between arrays (Aquantile method) and then use design <- modelMatrix(targets,ref="gDNA") to fit the linear model by lmFit. 

I get M-values, but how can I convert them into the absolute expression values, where do I get the expression values for the gDNA?

 

Thanks beforehand and again sorry for bothering

 

Best regards,

Irina

 

______________________________________

 

Irina Borodina

Ph.D. student

 

Center for Microbial Biotechnology

BioCentrum-DTU

Søltofts Plads, Building 223

DK-2800 Kgs Lyngby

DENMARK

Tel.+45 4525 2659

Fax:+45 4588 4148

www.cmb.dtu.dk

 


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