Hello arrayens,
 
I would like your opinion on a purpose of design:
 
First reflexion: I'm agree with you : One design must answer a biological question!!!!
 
My first design : 4 replicats with 2 factors  (time and treatement with oxydant ) on 2  levels:
hence 
one controle 6h
one treated 6h
 
one controle 24h
one treated 24h
 
for this design , i have a complete design which answer a lot of questions....
 
Second Reflexion : simplified design but less information?!!!!!
 
Now, I built a new design : with 2 kinds of cell: A ( normal cell) and B ( deficient cell with a mutation ).
 
A priori , I want to take the first type of design  i.e
 
cell A controle 6h
cell A treated with oxydant 6h
cell A controle 24h
cell A treated with oxydant 24h
 
 
cell B controle 6h
cell B treated with oxydant 6h
cell B controle 24h
cell B treated with oxydant 24h

 
In this case too , I have a big design which answer a lot of questions ( perhaps the type of questions that I would wonder in 5 years) ....
 
But in this crual world , the money is limited!!!!
 
Hence , I would reduced my design ( and reduced the number of interpretation in the future) to eliminate alls controle ( i think that it's crazy but i need your confirmation or infirmation )¸
that's this design:
 
A treated with oxydant 6h
A treated with oxydant 24h
 
B treated with oxydant 6h
B treated with oxydant 24h


 
In fact  , in  this case ,  Ican compare A et B treated 6h ,  cell A being the control for l'experience 6h , 
 
In this design , I'm intererest only at the _expression differentiel between A and B , with an oxydant at 6h and 24h ......
 
 
 
What do you think about this design ??
 
 
 
Best regards
 
 
Greg Voisin
Bioinformaticien 
Centre de recherche de l'Hotel Dieu de Montreal




		
---------------------------------


	[[alternative HTML version deleted]]

