Hi there
 
I“ve been thinking about gene expression in just one condition without
comparing to anything else. 
 
I explain better: I have data from an  affy array experiment using naive
tissue RNA, and I want to know which genes, out of the 30000 present in the
chip, are being expressed in this tissue.  
 
I would like to know  is this analysis is  possible, i mean not just
statistically but also if scientifically has any sense,
 
And if it is I would need some help
 
a) is it possible to use bioconductor and GCRMA analysis ? then,  anyone
knows a script or could guide me?
b) how many replicas  do we need? 
c) which is the cut off point?
 
Basically which are the pros and the cons of this kind of analysis?
 
thank you very much!
marta
 
Marta Agudo PhD
Departamento de Oftalmologķa
Facultad de Medicina
Campus Espinardo
30100 Murcia- Spain
Phone:+34 968363996
 

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