[BioC] DEseq error

[Vineeta singh [guest]]

Dear Simon Anders
Greetings !!
This is Vineeta Singh currently working on RNAseq data and got stuck in one error while using DEseq in estimateDispersion  step error is given below . Its been wonderfull  package but am unable to use it. Please help me in resolving this error.  I tried giving method type, fitType, sharingMode but all options failed. This data is simulated one in which “A” type are created as replicate and same with”B” type. 


 -- output of sessionInfo(): 

Error in glm.fit(x = numeric(0), y = numeric(0), weights = NULL, start = c(0.1,  : 
  object 'fit' not found
In addition: Warning messages:
1: In is.na(rows) : is.na() applied to non-(list or vector) of type 'NULL'
2: In glm.fit(x = numeric(0), y = numeric(0), weights = NULL, start = c(0.1,  :
  no observations informative at iteration 1
3: glm.fit: algorithm did not converge 


> sessionInfo()
R version 3.0.3 (2014-03-06)
Platform: x86_64-w64-mingw32/x64 (64-bit)

locale:
[1] LC_COLLATE=English_United States.1252  LC_CTYPE=English_United States.1252    LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C                           LC_TIME=English_United States.1252    

attached base packages:
[1] parallel  stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] DESeq_1.14.0       lattice_0.20-29    locfit_1.5-9.1     Biobase_2.22.0     BiocGenerics_0.8.0

loaded via a namespace (and not attached):
 [1] annotate_1.40.1      AnnotationDbi_1.24.0 DBI_0.2-7            genefilter_1.44.0    geneplotter_1.40.0  
 [6] grid_3.0.3           IRanges_1.20.7       RColorBrewer_1.0-5   RSQLite_0.11.4       splines_3.0.3       
[11] stats4_3.0.3         survival_2.37-7      tools_3.0.3          XML_3.98-1.1         xtable_1.7-3  


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