[BioC] Problem with PMcorrection and Summarization
Kaj Chokeshaiusaha [guest]
guest at bioconductor.org
Sun Jan 19 15:04:41 CET 2014
Dear R helpers,
I have tried separate performances of background correction and normalization step by step. After I acquired the background corrected Affybatch. I expressed it for normalization using a script provided elsewhere. Finally, I acquired a normalized data matrix. However, I need to perform PMcorrection and Summarization subsequently. Due to my limited knowledge in R, I totally have no idea how to do it (At my best, I can only follow the vignette straightforwardly.)
Could anyone please show me how can I perform PMcorrection and Summarization with the normalized data matrix and finally acquire expressionset?
Best Regards,
Kaj
-- output of sessionInfo():
R version 2.15.3 (2013-03-01)
Platform: x86_64-w64-mingw32/x64 (64-bit)
locale:
[1] LC_COLLATE=Thai_Thailand.874 LC_CTYPE=Thai_Thailand.874
[3] LC_MONETARY=Thai_Thailand.874 LC_NUMERIC=C
[5] LC_TIME=Thai_Thailand.874
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] affy_1.36.1 Biobase_2.18.0 BiocGenerics_0.4.0
loaded via a namespace (and not attached):
[1] affyio_1.26.0 BiocInstaller_1.8.3 preprocessCore_1.20.0
[4] zlibbioc_1.4.0
--
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