[BioC] Importing quantile normalized .txt file to limma for differential expression

[James W. MacDonald]

You will need to give more information than this if you want help. How 
are you pre-processing the data? Using R or something else? What have 
you tried, and what were the results? Note that you cannot import 
anything into limma - you import into R and then use limma to analyze 
the data.

Also, have you read the limma User's Guide? If not, you should, as 
probably 99.325% (just an estimate) of your questions are covered in 
that document.

http://www.bioconductor.org/packages/2.12/bioc/vignettes/limma/inst/doc/usersguide.pdf

Best,

Jim



On Tuesday, September 24, 2013 9:04:07 AM, Dhanjit [guest] wrote:
>
> I am using limma package for identifying differential expression of affymetrix .CEL file. I used quantile normalization method and saved the normalized data in .txt file. However, i am not able to import the .txt file into limma for differential expression.
>
> Kindly tell me the sequential procedure for identifying the differential expression of quantile normalized data.
>
>   -- output of sessionInfo():
>
> aa
>
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--
James W. MacDonald, M.S.
Biostatistician
University of Washington
Environmental and Occupational Health Sciences
4225 Roosevelt Way NE, # 100
Seattle WA 98105-6099