[BioC] [limma] [Rfit] [samr] Gene expression distribution using lmFit and eBayes
Jérôme Lane
jerome.lane at criucpq.ulaval.ca
Thu Nov 21 23:42:21 CET 2013
Hi,
The 3/4 of my microarray gene expressions have non normal distribution with
most of p-values after Shapiro test under 10x-5.
I tried linear ranked regression from rfit (no normality assumption for
residues) from Rfit package for adjustment of covariables + SAM (non
parametric) from samr package but results where not as biologically relevant
as lmFit + eBayes could provide.
I know that lmFit function can analyses gene expression not strictly normal,
but what is the limit ?
Is it statistically relevant to use lmFit + eBayes according to my data ?
Best regards,
Jérôme Lane
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