[BioC] Small RNA seq data analysis using DESeq
Vedran Franke
vfranke at bioinfo.hr
Wed Jun 19 20:44:34 CEST 2013
Dear Simon,
I have a question regarding the analysis of small RNAseq data using DESeq.
While counting the reads per loci I have weighted the reads by the
reciprocal of the places to which the read maps.
I was wondering whether it is still proper to use the negative binomial
test implemented in DESeq (after rounding the expression estimates) to
determine which loci are differentially expressed?
Best regards,
Vedran Franke
More information about the Bioconductor
mailing list