[BioC] IlluminaHumanMethylation450k.db: Missing probes in IlluminaHumanMethylation450kPROBELOCATION function?

Simone enomis.bioc at gmail.com
Mon Jul 1 19:35:42 CEST 2013


Hello!

A little question: I've got a table with beta values obtained by
Illumina's 450K BeadChip microarray and want to know for each probe
where in the gene it is located (mainly promoter region or gene body).
I found the IlluminaHumanMethylation450kPROBELOCATION function in the
IlluminaHumanMethylation450k.db package which seems to do what I want,
but not for all the probes.
In detail, I have got data for 473,029 probes, but the function only
returns values for 354,770 unique probes, so 118,259 ones are missing.
Is this expected behaviour? Should I use another package to get
location information for all the probes contained in my file?

And secondly: As the IlluminaHumanMethylation450k.db package seems to
be deprecated, on which build of the genome is the
IlluminaHumanMethylation450kPROBELOCATION information based? Because I
see that in the package there are some separate functions for build 36
respectively 37, but in the help of the function in question I can
only find the information that mappings are based on data of Illumina
from January 2011 (which should then be GRCh37, the build I have to
work with in this case, but however I am not completely sure as it
doesn't say it clearly).

Best regards,
Simone

> sessionInfo()
R version 3.0.1 (2013-05-16)
Platform: x86_64-pc-linux-gnu (64-bit)

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C
LC_TIME=en_US.UTF-8
 [4] LC_COLLATE=en_US.UTF-8     LC_MONETARY=en_US.UTF-8
LC_MESSAGES=en_US.UTF-8
 [7] LC_PAPER=C                 LC_NAME=C
LC_ADDRESS=C
[10] LC_TELEPHONE=C             LC_MEASUREMENT=en_US.UTF-8
LC_IDENTIFICATION=C

attached base packages:
[1] parallel  stats     graphics  grDevices utils     datasets
methods   base

other attached packages:
 [1] sqldf_0.4-6.4                         RSQLite.extfuns_0.0.1
 [3] chron_2.3-43                          gsubfn_0.6-5
 [5] proto_0.3-10                          RColorBrewer_1.0-5
 [7] illuminaHumanv2.db_1.18.0             IlluminaHumanMethylation450k.db_2.0.7
 [9] IlluminaHumanMethylation27k.db_1.4.7  org.Hs.eg.db_2.9.0
[11] RSQLite_0.11.4                        DBI_0.2-7
[13] AnnotationDbi_1.22.6                  affy_1.38.1
[15] Biobase_2.20.0                        BiocGenerics_0.6.0

loaded via a namespace (and not attached):
[1] affyio_1.28.0         AnnotationForge_1.2.1 BiocInstaller_1.10.2
IRanges_1.18.1
[5] preprocessCore_1.22.0 stats4_3.0.1          tcltk_3.0.1
tools_3.0.1
[9] zlibbioc_1.6.0



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