[BioC] miRNA analysis advice

[Steve Lianoglou]

Hi,

On Mon, Feb 25, 2013 at 10:25 AM, James W. MacDonald <jmacdon at uw.edu> wrote:
[snip]
> In general I like to see somewhere around 15-30 differentially expressed
> miRNA transcripts, as anything more can become quite intractable for the
> people I collaborate with. This is because each miRNA may target > 1000 mRNA
> species, so too many miRNAs and all of a sudden every gene might be a
> potential target.

... and don't forget to model competing endogenous RNA (ceRNA) effects!

-steve

-- 
Steve Lianoglou
Graduate Student: Computational Systems Biology
 | Memorial Sloan-Kettering Cancer Center
 | Weill Medical College of Cornell University
Contact Info: http://cbio.mskcc.org/~lianos/contact