[BioC] Using a countDataSet as input data in DESeq2
Natalia [guest]
guest at bioconductor.org
Tue Dec 3 09:07:53 CET 2013
Dear all,
This question seems so simple, but I'm struggling to get it right! How to use a countDataSet obtained with easyRNAseq as input data in DESeq2?
Thank you very much in advance!
Kind regards
> countDataSet
CountDataSet (storageMode: environment)
assayData: 17108 features, 2 samples
element names: counts
protocolData: none
phenoData
sampleNames: NS1gallusDNAmapped.bam NS2gallusDNAmapped.bam
varLabels: sizeFactor condition
varMetadata: labelDescription
featureData
featureNames: ENSGALG00000000003 ENSGALG00000000004 ... ENSGALG00000029181 (17108 total)
fvarLabels: disp_blind
fvarMetadata: labelDescription
experimentData: use 'experimentData(object)'
Annotation:
-- output of sessionInfo():
> sessionInfo()
R version 3.0.2 (2013-09-25)
Platform: x86_64-w64-mingw32/x64 (64-bit)
locale:
[1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United States.1252 LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C LC_TIME=English_United States.1252
attached base packages:
[1] parallel stats graphics grDevices utils datasets methods base
other attached packages:
[1] DESeq2_1.2.6 RcppArmadillo_0.3.920.3 Rcpp_0.10.6 easyRNASeq_1.8.3 ShortRead_1.20.0 Rsamtools_1.14.2
[7] GenomicRanges_1.14.3 DESeq_1.14.0 lattice_0.20-24 locfit_1.5-9.1 Biostrings_2.30.1 XVector_0.2.0
[13] IRanges_1.20.6 edgeR_3.4.1 limma_3.18.3 biomaRt_2.18.0 Biobase_2.22.0 genomeIntervals_1.18.0
[19] BiocGenerics_0.8.0 intervals_0.14.0
loaded via a namespace (and not attached):
[1] annotate_1.40.0 AnnotationDbi_1.24.0 bitops_1.0-6 DBI_0.2-7 genefilter_1.44.0 geneplotter_1.40.0 grid_3.0.2
[8] hwriter_1.3 latticeExtra_0.6-26 LSD_2.5 RColorBrewer_1.0-5 RCurl_1.95-4.1 RSQLite_0.11.4 splines_3.0.2
[15] stats4_3.0.2 survival_2.37-4 XML_3.98-1.1 xtable_1.7-1 zlibbioc_1.8.0
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