[BioC] question about package AgiMicroRna
    maryam shahmansouri [guest] 
    guest at bioconductor.org
       
    Tue Aug 20 14:14:09 CEST 2013
    
    
  
Hello
1)I need that to implement another normalization between arrays methods like GPA and vsn,LVS,CyclicLoess on the ddTGS(gTotalGeneSignal).
2)I am interest that to obtain an estimate of the gene signal using the RMA algorithm without the function rmaMicroRna ; First I read data into RGlist,then I get BG corrected  and normalized between arrays in limma, but I don't know how can to obtain the median of the replicated probes. 
Please help me.
Thanks
 -- output of sessionInfo(): 
R version 2.15.1 (2012-06-22)
Platform: i386-pc-mingw32/i386 (32-bit)
locale:
[1] LC_COLLATE=English_United States.1252 
[2] LC_CTYPE=English_United States.1252   
[3] LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C                          
[5] LC_TIME=English_United States.1252    
attached base packages:
[1] splines   stats     graphics  grDevices utils     datasets  methods  
[8] base     
other attached packages:
 [1] LVSmiRNA_1.6.0        vsn_3.24.0            MASS_7.3-23          
 [4] quantreg_5.02         SparseM_1.03          AgiMicroRna_2.6.0    
 [7] affycoretools_1.28.0  KEGG.db_2.7.1         GO.db_2.7.1          
[10] RSQLite_0.11.4        DBI_0.2-7             AnnotationDbi_1.18.4 
[13] preprocessCore_1.18.0 affy_1.34.0           limma_3.12.3         
[16] Biobase_2.16.0        BiocGenerics_0.2.0   
loaded via a namespace (and not attached):
 [1] affyio_1.24.0       annaffy_1.28.0      annotate_1.34.1    
 [4] BiocInstaller_1.4.9 biomaRt_2.12.0      Biostrings_2.24.1  
 [7] Category_2.22.0     gcrma_2.28.0        genefilter_1.38.0  
[10] GOstats_2.22.0      graph_1.34.0        grid_2.15.1        
[13] GSEABase_1.18.0     IRanges_1.14.4      lattice_0.20-21    
[16] RBGL_1.32.1         RCurl_1.95-4.1      stats4_2.15.1      
[19] survival_2.37-4     tools_2.15.1        XML_3.98-1.1       
[22] xtable_1.7-1        zlibbioc_1.2.0   
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