[BioC] [methylumi] concerning methylumi.bgcorr function and out-of-band intensities

[Hannah Elliott]

Dylan Aïssi <dylan.aissi at ...> writes:

> 
> Dear all,
> I would like to understand what really does the methylumi.bgcorr function
> with method='noob' but I can't find information about out-of-band
> intensities.
> 
> Does anyone know what is meant by out-of-band intensities?
> 
> Thank you.
> 
> Dylan Aïssi
> 
> PhD student
> INSERM UMRS 937
> Faculté de Médecine Pierre et Marie Curie
> 91 Boulevard de l'Hôpital
> 75013 Paris
> 
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> 
> 
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Dear Dylan,
The use of out of band intensities for background correction is described in 
detail in this paper: http://www.ncbi.nlm.nih.gov/pubmed/23476028.

Signal intensity is measured from type I green probes in the red channel & 
type I red probes in the green channel as an alternative method of deriving 
the background signal from the array.

I hope this helps answer your question.

Hannah Elliott
University of Bristol