[BioC] Normalization of Affymetrix microarray data with spike-in hybridization controls?

[Thornton, Matthew]

Hello,

I am new to bioconductor and I have a question about fitting and normalizing the spike-in hybridization controls. 

>From what I have read so far, it seems that intensity and concentration can be related by way of a langmuir isotherm.  The concentrations of the hybridization controls for Affymetrix GeneChips is a known quantity. Does anyone here fit the the intensities of the hybridization controls and then use the fit data to normalize between replicates - like a scale factor?

If so, do you have a procedure? If not, is there a reason that one should not do this?

Thanks

Matt