[BioC] How to identify DE genes by comparing two groups where one contains a single array data

[Lei Li [guest]]

Hi, 

I have two groups of affymetrix microarray data, one group contains more than 10 normal microarrays, while another group only have 1 cancer microarray. I wonder whether limma could be used to identify differential-expressed genes by comparing these two groups? I am concerned about the accuracy of limma since the variance of the cancer data could not be estimated due to the sample limitation. 

Thanks,

Lei 

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