[BioC] Create A Stranded probeAnno Object
Dario Strbenac
d.strbenac at garvan.org.au
Fri May 25 08:00:44 CEST 2012
Also worth describing in ?probeAnno
> probes
Error in validObject(object) :
invalid class “probeAnno” object: 1: Probe matches are not sorted (in
increasing order) by their middle position on chromosome chr1.- and possibly
others.
invalid class “probeAnno” object: 2: Probe matches are not sorted (in
increasing order) by their middle position on chromosome chr1.+ and possibly
others.
invalid class “probeAnno” object: 3: Probe matches are not sorted (in
increasing order) by their middle position on chromosome chr10.- and possibly
others.
... ...
Also, I need to write a workaround for segChrom for any chromosomes that don't
have probes on both strands (boutique design array).
... ...
Running 'segment' on chromosome chr12.+ ... complete
Running 'segment' on chromosome chr12.- ... complete
Running 'segment' on chromosome chr13.+Error in probeAnno[w] :
No mapping 'chr13.+.start' in this 'probeAnno' object.
> probes
A 'probeAnno' object holding the mapping between
reporters and genomic positions.
Chromosomes: chr1.- chr1.+ chr10.- chr10.+ chr11.- chr11.+ chr12.- chr12.+
chr14.- chr14.+ chr15.- chr15.+ chr16.- chr16.+ chr17.- chr17.+ chr18.- chr18.+
chr19.- chr19.+ chr2.- chr2.+ chr20.- chr20.+ chr21.- chr21.+ chr22.- chr22.+
chr3.- chr3.+ chr4.- chr4.+ chr5.- chr5.+ chr6.- chr6.+ chr7.- chr7.+ chr8.-
chr8.+ chr9.- chr9.+ chrX.- chrX.+ chrY.- chrY.+
Microarray platform:
Genome:
Gives a valid object, so segChrom should work with it.
- Dario
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