[BioC] pdInfoBuilder: coreMps
Mike Walter
michael_walter at email.de
Thu May 10 15:41:53 CEST 2012
Dear List,
I have a set of the new AraGene 1.1-st arrays and thus like to build a
corresponding pdInfo package. Anyway, I get an error while creating the
package. Please see code and error mesasge below. Obviously, the mps file
for the array can't be found, and I was unable to figure out how to specify
the file location in the new("AffyGenePDInfoPkgSeed", ... ) function.
Looking through the vignette, archive and help files I never came across the
mps message, so any suggestion on how to specify the file (which is also in
the working directory).
Thank you very much in advance,
Michael
baseDir = "F:/Auswertung/Array Annotationen/Affy"
> (pgf <- list.files(paste(baseDir,
"MO_AraGene-1_1-st-v1_strip_libraryfile", sep="/"), pattern = ".pgf",
full.names = TRUE))
[1] "F:/Auswertung/Array
Annotationen/Affy/MO_AraGene-1_1-st-v1_strip_libraryfile/AraGene-1_1-st.pgf"
> (clf <- list.files(paste(baseDir,
"MO_AraGene-1_1-st-v1_strip_libraryfile", sep="/"), pattern = ".clf",
full.names = TRUE))
[1] "F:/Auswertung/Array
Annotationen/Affy/MO_AraGene-1_1-st-v1_strip_libraryfile/AraGene-1_1-st.clf"
> (prob <- list.files(baseDir, pattern = "tair10.probeset.csv",full.names =
TRUE))
[1] "F:/Auswertung/Array
Annotationen/Affy/AraGene-1_1-st-v1.na32.tair10.probeset.csv"
> (trans = list.files(baseDir, pattern = "tair10.transcript.csv",full.names
= TRUE))
[1] "F:/Auswertung/Array
Annotationen/Affy/AraGene-1_1-st-v1.na32.tair10.transcript.csv"
> (core = list.files(paste(baseDir,
"MO_AraGene-1_1-st-v1_strip_libraryfile", sep="/"), pattern = ".mps",
full.names = TRUE))
[1] "F:/Auswertung/Array
Annotationen/Affy/MO_AraGene-1_1-st-v1_strip_libraryfile/AraGene-1_1-st.mps"
>
> seed <- new("AffyGenePDInfoPkgSeed",
+ pgfFile = pgf, clfFile = clf,
+ probeFile = prob, author = "Michael Walter",
+ transFile = trans,
+ email = "michael.walter at med.uni-tuebingen.de",
+ biocViews = "AnnotationData",
+ genomebuild = "TAIR10",
+ organism = "Arabidopsis", species = "Arabidopsis thaliana")
>
> makePdInfoPackage(seed, destDir = ".")
============================================================================
====
Building annotation package for Affymetrix Gene ST Array
PGF.........: AraGene-1_1-st.pgf
CLF.........: AraGene-1_1-st.clf
Probeset....: AraGene-1_1-st-v1.na32.tair10.probeset.csv
Transcript..: AraGene-1_1-st-v1.na32.tair10.transcript.csv
Core MPS....: coreMps
============================================================================
====
Parsing file: AraGene-1_1-st.pgf... OK
Parsing file: AraGene-1_1-st.clf... OK
Creating initial table for probes... OK
Creating dictionaries... OK
Parsing file: AraGene-1_1-st-v1.na32.tair10.probeset.csv... OK
Parsing file: coreMps... Fehler in file(file, "rt") : kann Verbindung nicht
öffnen
Zusätzlich: Warnmeldungen:
1: In is.na(x) :
is.na() auf nicht-(Liste oder Vektor) des Typs 'NULL' angewendet
2: In is.na(x) :
is.na() auf nicht-(Liste oder Vektor) des Typs 'NULL' angewendet
3: In file(file, "rt") :
kann Datei 'coreMps' nicht öffnen: No such file or directory
>
> sessionInfo()
R version 2.14.0 (2011-10-31)
Platform: i386-pc-mingw32/i386 (32-bit)
locale:
[1] LC_COLLATE=German_Germany.1252 LC_CTYPE=German_Germany.1252
[3] LC_MONETARY=German_Germany.1252 LC_NUMERIC=C
[5] LC_TIME=German_Germany.1252
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] pdInfoBuilder_1.20.0 affxparser_1.26.4 RSQLite_0.11.1
[4] DBI_0.2-5 oligo_1.18.1 oligoClasses_1.16.0
[7] Biobase_2.14.0
loaded via a namespace (and not attached):
[1] affyio_1.22.0 Biostrings_2.22.0 bit_1.1-8
[4] ff_2.2-6 IRanges_1.12.6 preprocessCore_1.16.0
[7] splines_2.14.0 tools_2.14.0 zlibbioc_1.0.1
>
--
Dr. Michael Walter
MFT Services
University of Tübingen
Calwerstr. 7
72076 Tübingen
Tel.: +49 7071 29 83210
Fax. + 49 7071 29 5228
web: www.mft-services.de
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